[A new balancer of chapped wing on chromosome 3 and a gathering line with double balancers of chapped wing (L) and Cy in Drosophila melanogaster.].
نویسندگان
چکیده
The balancers of Drosophila melanogaster are widely used in genetic research. By analyzing the phenotype of offspring from hybridization of chapped wing (L) mating with OR, 982p and e, respectively, we mapped the chapped wing mutation on chromosome 3 for the first time and demonstrated the chapped wing mutation as a new balancer of D. melanogaster with dominant wing nicking phenotype. Finally, we bred a novel gathering line with double balancers of L and Cy in D. melanogaster. The mutant L provided a legible dominant marker for the balancer of chromosome 3, and the cultivation of double balancers chapped-curly wing enriches the balancer stock, which is often used in mapping and screening.
منابع مشابه
The Molecular and Genetic Characterization of Second Chromosome Balancers in Drosophila melanogaster
Balancer chromosomes are multiply inverted and rearranged chromosomes used in Drosophila melanogaster for many tasks, such as maintaining mutant alleles in stock and complex stock construction. Balancers were created before molecular characterization of their breakpoints was possible, so the precise locations of many of these breakpoints are unknown. Here, we report or confirm the positions of ...
متن کاملThird Chromosome Balancer Inversions Disrupt Protein-Coding Genes and Influence Distal Recombination Events in Drosophila melanogaster
Balancer chromosomes are multiply inverted chromosomes that suppress meiotic crossing over and prevent the recovery of crossover products. Balancers are commonly used in Drosophila melanogaster to maintain deleterious alleles and in stock construction. They exist for all three major chromosomes, yet the molecular location of the breakpoints and the exact nature of many of the mutations carried ...
متن کاملRab11 is required for maintenance of cell shape via βPS integrin mediated cell adhesion in Drosophila
In eukaryotes, vesicle trafficking is regulated by the small monomeric GTPases of the Rab protein family. Rab11, (a subfamily of the Ypt/Rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric Rab GTPases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. In an earlier communication, we have shown th...
متن کاملGFP-tagged balancer chromosomes for Drosophila melanogaster
We constructed green fluorescent protein (GFP)-expressing balancer chromosomes for each of the three major chromosomes of Drosophila melanogaster. Expression of GFP in these chromosomes is driven indirectly by a Kruppel (Kr) promoter, via the yeast GAL4-UAS regulatory system. GFP fluorescence can be seen in embryos as early as the germ band extension stage, and can also be seen in larvae, pupae...
متن کاملInduction of somatic mutation and recombination by four inhibitors of eukaryotic topoisomerases assayed in the wing spot test of Drosophila melanogaster.
Four inhibitors of eukaryotic topoisomerases were investigated for genotoxic effects in the wing spot test of Drosophila melanogaster. As a somatic mutation and recombination test (SMART) this assay assesses mitotic recombination and mutational events of various kinds. We studied camptothecin as a topoisomerase I inhibitor, as well as ellipticine as an intercalating inhibitor and teniposide and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Yi chuan = Hereditas
دوره 32 10 شماره
صفحات -
تاریخ انتشار 2010